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Dianova公司為德國(guó)的病理免疫組化產(chǎn)品供應(yīng)商，具備的抗體制備工藝及嚴(yán)格的質(zhì)控系統(tǒng)，產(chǎn)品在上享有良好的聲譽(yù)。

克隆號(hào)為H09的IDH1（isocitrate dehydrogenase 1, 異檸檬酸鹽脫氫酶1）鼠單克隆抗體為Dianova公司近期推出的產(chǎn)品之一，此抗體可特異性的檢測(cè)腦組織腫瘤中IDH1基因的R132H點(diǎn)突變。IDH1基因的第132密碼子的雜合突變是II 和III級(jí)神經(jīng)膠質(zhì)瘤（根據(jù)WHO的腫瘤學(xué)分類）的頻發(fā)事件。在大約70％的星形膠質(zhì)細(xì)胞瘤和少突神經(jīng)膠質(zhì)細(xì)胞瘤可發(fā)現(xiàn)IDH1基因的R132H點(diǎn)突變。

IDH1基因R132H突變?cè)诓糠帜X腫瘤中的頻發(fā)現(xiàn)象使克隆號(hào)為H09的IDH1抗體可高靈敏性和高特異性的用于此類腫瘤的鑒別診斷中：如從原發(fā)性膠質(zhì)母細(xì)胞瘤中區(qū)分出間變性星形細(xì)胞瘤、從毛細(xì)胞型星形細(xì)胞瘤或室管膜（細(xì)胞）瘤中區(qū)分出彌漫性星形細(xì)胞瘤（WHO分類II級(jí)），也可以用于具有IDH1突變的腫瘤細(xì)胞浸潤(rùn)前沿的與反應(yīng)性神經(jīng)膠質(zhì)增多癥的區(qū)分。因此，由于其高靈敏性和高特異性，此抗體在腦部腫瘤的分類分級(jí)中以及在檢測(cè)單一或少量浸潤(rùn)腫瘤細(xì)胞時(shí)是非常有用的。

DIA-808 – Anti-CD8a (Ms) from Rat (Clone: GHH8) for mouse FFPE tissue – 200 µl

Art.-Nr.                          DIA-808

Specificity                       CD8a (MHC I Co-Receptor, cytotox. T-cells)

Species Reactivity                Mouse

Host Species                       Rat

Isotype                            IgG2a/k

Clone                              GHH8

Clonality (Mono-/Polyclonal)       monoclonal

Application                       Immunohistochemistry (Paraffin-embedded Sections)

Conjugation                        unconjugated

Format                            lyophilisate

                                  antibody purified (from culture supernatant)

Product line / Topic               CD markers

                                   immunohistochemistry

Intended Use                        for Research Use Only

Search Code                         DIA808

Manufacturer / Brand                dianova

Reactivity:

Antibody clone GHH8 has been developed and validated for the detection of murine CD8a in formalin-fixed paraffin-embedded tissue sections (mouse FFPE). Clone GHH8 identifies cytotoxic/suppressor T-cells in murine FFPE tissue by IHC.
CD8 is a marker for T-cells with suppressor and cytotoxic activity. CD8 is also found on the surface of some natural killer cells, most thymocytes, some T-cell lymphomas and large granular lymphocyte leukemias.
Antigen recognition and subsequent T-cell activation is controlled by the interaction between TCR (T-cell receptor) and pMHC (peptide-major histocompatibility complexe). T-cell activation is often enhanced by a close cooperation of the TCR with two dif-ferent co-receptors: CD8 and CD4. CD8 was first discovered as a cell surface marker in mice and was used to discriminate CD8+ CTLs and CD4+ Th cells. CD8a is expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). CD8 alpha beta is expressed on the majority of cytotoxic T-lymphocytes (CTLs). This CD8 heter-omeric proteins (with approx. 32-34 KDa each monomer) are physically associated with a p56 tyrosine kinase to mediate T-cell activation signals through phosphorylation of adjacent proteins.
Besides its important role in T-cell signalling, the CD8 co-receptor is also important for CD8+ T-cell development, stabilisation of the interface between CD8+ T-cells and target cells, and activation of intestinal epithelial T lymphocytes (iIELs). Therefore, CD8 has been the target of many therapeutic strategies designed to modulate T-cell responses during auto-immune reactions, allergy and organ transplant rejection.

Immunohistochemistry of mouse CD8a in formalin-fixed paraffin-embedded tissue sections

A, Mouse spleen: The monoclonal antibody clone GHH8 specifically stains CD8a in standard formalin-fixed paraffin-embedded mouse spleen. Incubation with antibody clone GHH8 (5μg/ml, corresponding to a 1:100 dilution) for 1h at RT. Detection with Im-mPRESS HRP anti-Rat from Vector Laboratories (Picture courtesy of Chris Bonnas, Synaptic Systems, Göttingen, Germany).
B, Mouse liver after viral infection: Specific Detection of invading T-cells with monoclonal antibody clone GHH8 by IHC-FFPE. Note the clear membrane-bound signal free of background (left picture). Detection with a Ventana automated stainer following standard procedures. Incubation for 30min at 4°C with 10μg/ml of antibody clone GHH8, corresponding to a 1:50 dilution (Pic-ture courtesy of Susanne Krasemann, Mouse Pathology Core Facility, UKE, Hamburg, Germany).

A

B

References

1. Kersh AE et al. Cutting Edge: Resident Memory CD8 T Cells Express High-Affinity TCRs. Journal of Immunology 2015, 195: 3520-4.
2. Wu RC et al. New insights on the role of CD8(+)CD57(+) T-cells in cancer. Oncoimmunology 2012, 1: 954-56.
3. Hyland L et al. Mice lacking CD8+ T cells develop greater numbers of IgA-producing cells in response to a respiratory vi-rus infection. Virology 1994, 204: 234-41.
4. Prince HE et al. CD4 and CD8 subsets defined by dual-color cytofluorometry which distinguish symptomatic from asymp-tomatic blood donors seropositive for human immunodeficiency virus. Diagnostic & Clinical Immunology 1987, 5: 188-93.
5. Gaudernack G et al. Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies di-rectly conjugated to monosized magnetic microspheres. Journal of Immunological Methods 1986, 90: 179-87.
6. Fleischer B et al. Function of the CD4 and CD8 molecules on human cytotoxic T lymphocytes: regulation of T cell trigger-ing. Journal of Immunology 1986,136: 1625-8.

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