Hamilton Company專注于精密測(cè)量設(shè)備，自動(dòng)化液體處理工作站和樣品管理系統(tǒng)的開(kāi)發(fā)，制造和定制。Hamilton的流程針對(duì)質(zhì)量和靈活性進(jìn)行了優(yōu)化。無(wú)論是具有快速交付時(shí)間框架的定制針頭，特殊長(zhǎng)度的pH傳感器，還是全面自動(dòng)化您的檢測(cè)工作流程的全面解決方案，相信Hamilton產(chǎn)品將始終滿足您的需求。
Hamilton公司60多年來(lái)一直是的制造商，總部位于內(nèi)華達(dá)州里諾市; 馬薩諸塞州富蘭克林; 蒂米什瓦拉，羅馬尼亞; 和瑞士的Bonaduz; 和遍布的子公司。
Hamilton為PRP-X100陰離子交換HPLC色譜柱提供了一種快速簡(jiǎn)便的方法來(lái)制備4.0 mM對(duì)羥基苯甲酸/ 2.5％甲醇，pH 8.5流動(dòng)相。
零件號(hào)/ REF | 描述 |
79325 | Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶1瓶） |
79335 | Eluent Concentrate，PRP-X100陰離子交換，（60 mL瓶裝，每瓶6瓶） |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-1 | PSDVB* | N/A | 100 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-C18 | C18 bonded to PSDVB* | N/A | 100 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-3 | PSDVB* | N/A | 300 Å | 5-85 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-h5 | Fluorinated PSDVB* | N/A | 300 Å | 5-85 °C | pH 1 – 13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HxSil C18 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
HxSil C8 | Silica | N/A | 110 Å | 5-60 °C | pH 1.5 – 10. 0-100% aqueous, organic modifier | N/A | 6,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X100 | PSDVB* with Tri- methyl ammonium Exchanger | 0.19meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X110 | PSDVB* with Tri-methyl ammonium Exchanger | 0.11meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X500 | Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-2.0N | 5,000 psi |
PRP-X600 | Poly (di-methyl amido-propyl meth-acrylamide) | 1.6meq/gm | Superficially porous | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-10 | PSDVB* with Tri-methyl ammonium Exchanger | 0.35meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
RCX-30 | PSDVB* with Tri-methyl ammonium Exchanger | 1.0meq/gm | 100 Å | pH Dependent** | pH 1-13. 0-100% aqueous, organic modifier | 0.0-1.0N | 5,000 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X200 | PSDVB* Sulfonate Exchanger | 35ueq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
PRP-X400 | PSDVB* Sulfonate Exchanger | 2.5meq/gm | N/A | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-5.0N | 5,000 psi |
PRP-X800 | PSDVB* Itaconate Exchanger | 1.6meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
HC-40 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water | Water | 1,000 psi |
HC-75 Calcium | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Hydrogen | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
HC-75 Lead | PSDVB* Sulfonate Exchanger | 5meq/gm | Gel-type | 25-90 °C | 100% Water 0-40% Acetonitrile | Water | 400 psi |
Packing Name | Support Material | Exchange Capacity | Pore Size | Temp. Limits | Phase Limits | Buffer Strength | Maximum Pressure |
PRP-X300 | PSDVB* Sulfonate Exchanger | 0.17meq/gm | 100 Å | 5-60 °C | pH 1-13. 0-100% aqueous, organic modifier | 0.0-0.5N | 5,000 psi |
*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C
HPLC柱具有有限的壽命。建議您使用標(biāo)準(zhǔn)定期監(jiān)測(cè)色譜柱的保留特性和性能。如果您打算長(zhǎng)時(shí)間儲(chǔ)存??色譜柱，請(qǐng)使用抑制微生物生長(zhǎng)的流動(dòng)相（含有sodium azide或高濃度甲醇，乙腈等的流動(dòng)相）。為獲得適性能和色譜柱壽命，請(qǐng)遵循以下建議。
恢復(fù)色譜柱性能
下面列出的步驟可能會(huì)恢復(fù)已經(jīng)污染的色譜柱的性能，但這些過(guò)程不會(huì)改善損壞或超出其使用壽命的色譜柱的性能。也不能恢復(fù)所有固定相。PRP-X500和PRP-X600色譜柱的*支持使得無(wú)法通過(guò)洗滌程序恢復(fù)性能。有關(guān)這些色譜柱的技術(shù)幫助，請(qǐng)HPLC支持。注意：在執(zhí)行恢復(fù)程序之前，請(qǐng)務(wù)必確保流動(dòng)相是可混溶的，并且不會(huì)發(fā)生沉淀。如有必要，在轉(zhuǎn)換到新的流動(dòng)相之前，先用合適的中間溶劑清洗色譜柱。應(yīng)使用至少五個(gè)柱體積的中間溶劑。
色譜柱修復(fù)程序
按照下面列出的適當(dāng)修復(fù)程序，使用1.0或2.0 mL / min的流速。除非另有規(guī)定。使用每列隨附的性能測(cè)試報(bào)告中列出的測(cè)試混合物和條件評(píng)估色譜柱性能。
柱修復(fù)程序
Packing Material Name
| Restoration Procedure |
PRP-C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-1 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-3 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-h5 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C8 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
HxSil C18 | Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes. Equilibrate with starting phase for 5-10 column volumes. |
PRP-X100 | Pump approximay 50 mL of methanol with 1% 6 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X200 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X300 | Inject several times with 100 µL of 1 N nitric acid. Equilibrate with starting phase for ~ 200 column volumes. |
PRP-X400 | Inject Several times with 100 µL of 0.1 M potassium EDTA. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-10 | Flush with 50 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
RCX-30 | Flush with 150 mL 0.1 N sodium hydroxide. Equilibrate with starting phase for ~ 200 column volumes. |
HC-40 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
HC-75 | Ca2+ Flush with 1% calcium chloride at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| H+ Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
| Pb2+ Flush with 1% lead nitrate at 0.1 mL/min overnight. Equilibrate with starting phase for ~ 200 column volumes. |
零件號(hào)/ REF | 描述 |
79641 | 分析型HPLC色譜柱，CUSTOM ORDER（1.0,2.1,4.1,4.6 mm ID） |
79669 | 負(fù)離子快 |
79670 | 負(fù)離子微 |
79668 | 負(fù)離子分辨率 |
79643 | 制備型HPLC色譜柱＃1，定制（21.2,30 mm內(nèi)徑） |
79644 | 制備型HPLC色譜柱＃2，定制（50 mm內(nèi)徑） |
79645 | 制備型HPLC色譜柱＃3，CUSTOM ORDER（100 mm ID） |
79421 | PRP-X10010μm2.1x 150 mm |
79346 | PRP-X10010μm2.1x 250 mm |
79439 | PRP-X10010μm4.1x 100 mm |
79434 | PRP-X10010μm4.1x 150 mm |
79433 | PRP-X10010μm4.1x 250 mm |
79365 | PRP-X10010μm4.1x 50 mm |
79354 | PRP-X10010μm4.6x 150 mm PEEK |
79455 | PRP-X10010μm4.6x 250 mm PEEK |
79353 | PRP-X10012-20μm100Å21.2x 250 mm |
79852 | PRP-X1005μm2.1x 150 mm PEEK |
79190 | PRP-X1005μm2.1x 250 mm |
79538 | PRP-X1005μm4.1x 100 mm |
79812 | PRP-X1005μm4.1x 150 mm |
79810 | PRP-X1005μm4.1x 50 mm |
79174 | PRP-X1005μm4.6x 150 mm PEEK |
79181 | PRP-X1005μm4.6x 250 mm PEEK |
79665 | PRP-X1007μm4.6x 150 mm PEEK |
79642 | 半制備型HPLC色譜柱，CUSTOM ORDER（7.0,7.8,10 mm ID） |
貨號(hào) | 品名 |
79477 | Analytical Guard Cartridge Holder, PEEK |
32908 | Analytical Guard Cartridge Holder, Stainless Steel |
79293 | Analytical Guard Column, PRP-X110 |
79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
79732 | PRP-X110 7 µm 4.1 x 150 mm |
79734 | PRP-X110 7 µm 4.1 x 250 mm |
79738 | PRP-X110 7 µm 4.6 x 150 mm PEEK |
79729 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
79728 | PRP-X110 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
79727 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
79726 | PRP-X110 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
79827 | PRP-X110, 7 µm Bulk Resin |
79743 | PRP-X110S 7 µm 2.1 x 100 mm PEEK |
79733 | PRP-X110S 7 µm 4.1 x 150 mm |
79735 | PRP-X110S 7 µm 4.1 x 250 mm |
79741 | PRP-X110S 7 µm 4.6 x 250 mm PEEK |
79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |
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